CT Features of Invasive Hydatidiform Mole / 实用放射学杂志

Objective To study the CT features of invasive hydatidiform mole and CT value in clinical application. Methods Thirteen cases of invasive hydatidiform mole proved by clinic and pathology were examined with conventional CT and contrast CT, then the CT features were analysed . Results CT findings of invasive hydatidiform mole included : enlargement of uterus, multiple hypodense cysts of different sizes and isodense soft tissue inside uterus, hypodense cysts inside the myometrium thicken irregularly,the cyst wall and isodense soft tissue enhanced markedly and up to 30~50 HU.Conclusion The CT features of invasive hydatidiform mole are specific and CT is of important value in clinical application.

Effects of apolipoprotein A-Ⅰon ATP binding cassette transporter A1 degradation and cholesterol efflux in THP-1 macrophage-derived foam cells / 中国病理生理杂志

AIM: To study the effects of apolipoprotein (apo) A-Ⅰon ATP binding cassette transporter A1 (ABCA1) degradation and cholesterol efflux in THP-1 macrophage-derived foam cells. METHODS: After exposure of the cultured THP-1 macrophage-derived foam cells to apolipoprotein A-Ⅰ for different time, cholesterol efflux, ABCA1 mRNA and protein level were determined by liquid scintillation counting, reverse transcriptase-polymerase chain reaction and Western blotting, respectively. The mean ABCA1 fluorescence intensity on THP-1 macrophage-derived foam cells was detected by flow cytometry. RESULTS: ApoA-Ⅰ markedly increased ABCA1-mediated cholesterol efflux from THP-1 macrophage-derived foam cells. This was accompanied by an increase in the content of ABCA1. ApoA-Ⅰ did not alter ABCA1 mRNA abundance. Thiol protease inhibitors increased the level of ABCA1 protein and slowed its decay in THP-1 macrophage-derived foam cells, whereas none of the proteosome-specific inhibitor lactacystin, other protease inhibitors, or the lysosomal inhibitor NH_4Cl showed such effects. The apoA-Ⅰ mediated cellular cholesterol efflux was enhanced by thiol protease inhibitors. CONCLUSION: Thiol protease inhibitors might provide an alternative way to upregulate ABCA1 protein. This strategy is especially appealing since it may mimic the stabilizing effect of the natural ligands apoA-Ⅰ.